Bacterial Expression Vector Flag Tag

Several vectors containing the t7 promoter offer dual tag options for flag and mat tagged fusion proteins.

Bacterial expression vector flag tag. T7 plasmid expression system features bacterial t7 promoter based vectors allow for expression detection and purification of recombinant flag and mat metal affinity tag fusions in e. Several vectors containing the t7 promoter offer dual tag options for flag and mat tagged fusion proteins. Vectors have an enterokinase cleavage site for removal of the tag from purified protein. Also offered are t7 promoter based bacterial expression vectors which allow for expression detection and purification of recombinant flag and mat metal affinity tag fusions in e.

The pcmv flag mat tag 1 expression vector is a shuttle vector containing both bacterial and sv40 origins of replication for propagation in both e. This pack enables you to compare placing flag epitope tags at either the n or c terminus of your gene of interest inserted into the mcs under transcriptional control of the oxb20 strong bacterial promoter with and also without a tev tobacco etch virus protease cleavage site. To clone into this vector add lic fusion tags to the 5 end of your pcr primers. This plasmid is an empty vector to be used with a lic cloning protocol.

Efficiency of replication in mammalian cells is optimal when using host cells that express the sv40 large t antigen. Includes sites for lic cloning and a stuffer fragment that includes the sacb gene allowing for negative selection on 5 sucrose. Other empty vectors for protein purification from the structural genomics consortium and nicola burgess brown containing a variety of tags can be found. It has a tev cleavable flag fusion tag on its n terminus.

Atum offers rhamnose iptg t5 and t7 and phoa based inducible bacterial expression vectors. The flag and 3xflag vectors are available for bacterial and mammalian expression using t7 tac or cmv promoters. Together with c myc some vectors offer dual labeling. Pgex expression vector with n terminal gst tag and tev cleavage site.

Removal of the flag tag is possible using enterokinase which cleaves following the asp asp asp asp lys. Combining these with different strength ribosome binding sites and origins of replication provides an excellent range of induced and uninduced expression levels.